Induction and inhibition of bovine leukaemia virus expression in naturally infected cells

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Induction and inhibition of bovine leukaemia virus expression in naturally infected cells.

Bovine leukaemia virus (BLV) resides in infected lymphocytes in a latent, repressed state but becomes expressed a few hours after the cells are cultured in vitro. We have identified several conditions and factors affecting the expression of BLV in short-term cultures of naturally infected lymphoid cells. The presence of foetal calf serum in the culture medium greatly stimulates virus expression...

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Α-lactalbumin Gene and Susceptibility of Lymphocytes to Infections with Bovine Leukaemia Virus and to Apoptosis in Naturally Infected Cows

A study was undertaken to analyse the effect of polymorphism of α-lactalbumin (ALA) gene at position -1689 on the susceptibility of lymphocytes to the infection with bovine leukaemia virus (BLV) and to undergoing apoptosis during the first three months after calving. The experiments covered 97 Black-and-White breed cows, aged 3–8 years, from three herds. The infection with BLV was diagnosed on ...

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FLOW CYTOMETRY DETERMINATION OF BCl-2 EXPRESSION IN ANIMALS INFECTED WITH BOVINE LEUKAEMIA VIRUS

Investigations were performed in lambs experimentally infected with blood lymphocytes of leukaemic cow. The presence of specific bovine leukaemia virus (BLV) antibodies in sera of infected animals was detected in agar gel immunodiffusion test and ELISA. Proviral DNA was detected with PCR and nested PCR. Dual-colour flow cytometry analysis with the use of specific monoclonal antibodies (for lymp...

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Bovine leukaemia virus and enzootic bovine leukosis.

Infection of bovines with bovine leukaemia virus (BLV) manifests itself in either of two ways: 30-70% of carriers develop persistent lymphocytosis (PL), with the viral genome integrated at a large number of different sites in the DNA of the affected B-lymphocytes, without causing any chromosomal abnormalities. Only 0,1-10% of carriers develop lymphoid tumours, which also consist of B-lymphocyte...

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Bovine Leukaemia Virus onco-protein Tax antigen identification in human lymphomas tissue

Background: Bovine leukaemia virus (BLV) is the primary cause of enzootic bovine leukaemia which belongs to the HTLV/BLV group of oncogenic retroviruses including Human T-cell leukaemia virus types 1, 2, and 3 and simian T-lymphotropic virus. Due to daily consumption of milk, meat and other dairy by-products, a possible role in appearing human cancers can be assumed for BLV. Materials and Metho...

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ژورنال

عنوان ژورنال: Journal of General Virology

سال: 1992

ISSN: 0022-1317,1465-2099

DOI: 10.1099/0022-1317-73-8-1915